ABSTRACT
The TIPE( tumor necrosis factor-alpha-induced protein 8-like) family has been recently described as regulators of tu-morigenesis and inflammation .The family consists of four highly homologous members: TNFAIP8 ( tumor necrosis factor-α-induced protein 8), TIPE1 (TNFAIP8L1), TIPE2 (TNFAIP8L2) and TIPE3 (TNFAIP8L3).Although TNFAIP8 family share high degrees of sequence homology , the members have different histological expressions , biological functions and molecular targets .TNFAIP8 shows the functions of inhibiting bacterial infection and promoting tumor migration .As a negative regulator of immunity and inflammation , TIPE2 is also an inhibitor of the oncogenic Ras in some neoplastic diseases .TIPE1 can induce cell apoptosis and inhibit tumor .TIPE3 is the transfer protein of phosphoinositide second messengers and can promote cancer .Emerging studies show TIPE family play important regulatory roles in many diseases;however, specific biological activities and exact molecular mechanisms need to be further elucidated .
ABSTRACT
Objective:To explore if there was a correlation between TNFAIP8 and gastric tumor drug resistance. Methods:We collected 47 cases of neoadjuvant chemotherapy,and detected the expression of TNFAIP8 in their tissues before and after surgery by im-munohistochemistry. Compared the expression of TNFAIP8 between SGC7901 cell and its cisplatin resistance cell SGC7901-DDP by Western blot,and then we used siRNA technology to decreased its expression,continued to test the susceptibility to cisplatin through MTT. Results:The expression of TNFAIP8 between pre-neoadjuvant and after-neoadjuvant had no difference, but the expression of chemotherapy effectiveness was lower than chemotherapy invalidness(P<0. 05);the expression of SGC7901-DDP cell was also higher than SGC7901 cell; the expression of TNFAIP8 decreased apparently through plasmid transfection of SGC7901-DDP cell, MTT test showed the fewer expression of TNFAIP8 could increase the susceptibility to cisplatin(P<0. 05). Conclusion: TNFAIP8 was involved in chemotherapy resistance of gastric carcinoma.
ABSTRACT
Objective:To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-pSIREN-RetroQ.Methods:Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-pSIREN-RetroQ interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results:We successfully design and built three TNFAIP8-shRNA-pSIREN-RetroQ interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.